Differential manifestation of RONS and antioxidant enzymes in response to singular versus combinatorial stress in Chironomus ramosus

Abstract In nature, organisms face multiple abiotic stress concurrently. Our previous study has indicated how threshold level of lethality depends on the type and combination stressors. Many mechanisms exist by which respond to stressors maintain homeostasis. We examined homeostatic pliability in an extremophilic oriental midge Chironomus ramosus larvae under various combinatorial conditions desiccation (DS), heat (HS) starvation (SS). Exposure these led activation a common response pathway oxidative stress. Abundance antioxidant enzymes like superoxide dismutase, catalase, glutathione reductase peroxidase along with selective as well stressor specific increase total capacity were reflected from corresponding reactive oxygen nitrogen species (RONS) exposed Additionally, we found increment lipid peroxidation level, protein carbonyl content advanced products during regime. Further investigation revealed sharp decline activity mitochondrial aconitase enzyme induced The comparative based biochemical fluorescence redox-endpoint assays confirmed that generation is consequential convergent DS, HS SS, but quantum RONS decides redox potential survival rate.